The Nuclease that cleaves all forms of DNA and RNA
DENARASE® is a genetically engineered endonuclease from Serratia marcescens used as a DNA eraser in purification processes of biological molecules. The enzyme cleaves all forms of DNA and RNA into smaller nucleotides of around 5-8 base pairs.
The enzyme is active in a broad range of conditions and is free of proteolytic activity. This makes the enzyme especially useful for applications with challenging DNA contents such as lysed host cells in bioprocessing.
Because of its high efficacy, enzymatic digestion is a preferred method for reducing DNA levels well below the limits for biologicals set by the authorities.
The patented production process uses a Bacillus sp. production host. The gram-positive, endotoxine-free strain is highly productive and mitigates the risk of endotoxins in the final product. DENARASE has a purity of ≥ 99 % and is manufactured in full compliance with GMP requirements using only raw materials of non-animal origin and without the use of antibiotics.
DENARASE is used especially in the field of:
- Gene Therapy
- Cell Therapy
- Viral Vectors