DENARASE® is a genetically engineered endonuclease from Serratia marcescens and used for DNA removal applications in GMP manufacturing processes of biologicals.
Alternative DNA removal technologies such as precipitation often do not provide sufficient product quality. Endonucleases only use nucleic acids as a substrate and do not interact with proteins. Moreover, they are very active under conditions that are favorable for biomolecules, which assures a high product quality and yield.
Enzymatic degradation of DNA can be useful in any kind of application where biomolecules are exposed to DNA such as:
- Viruses and virus like particles for vaccines
- Viral vectors, such as AAV and Lentivirus for gene and cell therapies, and oncolytic vaccines
- Viscosity reduction in lysates
- Sample preparation in electrophoresis and chromatography
How it works
DENARASE® specifically hydrolyzes the phosphodiester bonds between nucleotides leaving smaller fragments of around 5 – 8 base pairs. The enzyme is active on all forms of nucleic acids including single-stranded, double-stranded, linear, circular or supercoiled. Best results are obtained when the enzyme is added before the release of DNA.
Production of DENARASE®
DENARASE® has been developed for use in commercial manufacturing processes of biologicals and complies with EU GMP regulations. The patented production process uses a gram-positive Bacillus sp. production host, which minimizes the risks of endotoxins in the product. No antibiotics, materials with TSE/BSE risk or raw materials of animal origin are employed in the manufacture of the product.