For enzymatic DNA removal in Bioprocessing!


DENARASE® is a genetically engineered endonuclease from Serratia marcescens and used for DNA removal applications in GMP manufacturing processes of biologicals.

Alternative DNA removal technologies such as precipitation often do not provide sufficient product quality. Endonucleases only use nucleic acids as a substrate and do not interact with proteins. Moreover, they are very active under conditions that are favorable for biomolecules, which assures a high product quality and yield.


Enzymatic degradation of DNA can be useful in any kind of application where biomolecules are exposed to DNA such as:

  • Viruses and virus like particles for vaccines
  • Viral vectors, such as AAV and Lentivirus for gene and cell therapies, and oncolytic vaccines
  • Viscosity reduction in lysates
  • Sample preparation in electrophoresis and chromatography

How it works

DENARASE® specifically hydrolyzes the phosphodiester bonds between nucleotides leaving smaller fragments of around 5 ­– 8 base pairs. The enzyme is active on all forms of nucleic acids including single-stranded, double-stranded, linear, circular or supercoiled. Best results are obtained when the enzyme is added before the release of DNA.

Production of DENARASE®

DENARASE® has been developed for use in commercial manufacturing processes of biologicals and complies with EU GMP regulations. The patented production process uses a gram-positive Bacillus sp. production host, which minimizes the risks of endotoxins in the product. No antibiotics, materials with TSE/BSE risk or raw materials of animal origin are employed in the manufacture of the product.

Not quite ready to buy yet?

  • Get your free sample DENARASE®!

    New customers from the pharmaceutical industry can request a free 25 kU sample for first lab trials. Please fill out the request form to get your free sample.

  • ELISA-Kit for analytical support

    Learn more about our ELISA Kit

    For the quantitative analysis of endonucleases from Serratia marcescens a DENARASE ELISA detection kit is available.

    learn more

Get in touch!

Speak to a member of our expert team and learn more about our DENARASE products!

Qualified and used
by big pharma companies

  • „We have used DENARASE in our manufacturing production. It is good according to our test. We plan to use it in next valuable schedule.”

    US-Children Hospital, USA,
    Clinical Vector Core, Lead Scientist
  • „I tested the DENARASE yesterday and it was equally good as our standard in my setting. I will continue using it in variety of conditions that I normally use and I will most certainly be purchasing my future enzymes from you.”

    US University, USA,
  • „We are going to use DENARASE exclusively and no longer our usual nuclease. The product is excellent in performance and price.”

    US biopharmaceutical manufacturer, USA,
    Lab Manager Vector Production