Viral Vector Production for Gene Therapy
Gene therapies make use of genetic material like DNA or RNA, which is introduced into cells inside the human body to treat diseases. Viral vectors such as Adeno Associated Vectors (AAV) and lentiviruses are the preferred vehicles for DNA transfer into cells.
Host expression systems such as HEK293 cells are used for viral vector manufacturing. The genetic information for the virus and the therapeutic gene is introduced into the culture using plasmids. After viral vector production the cells need to be lysed for viral vector yield optimization. At this stage the product is exposed to large quantities of nucleic acids from the host cell and residual plasmids.
DNA can form aggregates with product and cell debris, which makes viral vector purification challenging. Addition of DENARASE before the lysis step rapidly digests DNA without interacting with the product. This helps to improve performance of subsequent purification steps and increases the overall vector yield.