For enzymatic DNA removal in Bioprocessing!

Gene Therapy

Gene therapies make use of genetic material like DNA or RNA, which is introduced into cells inside the human body to treat diseases. Viral vectors such as Adeno Associated Vectors (AAV) and lentiviruses are the preferred vehicles for DNA transfer into cells.

These viral vectors are produced in host expression systems such as HEK293 cells. The genetic information for the virus and the therapeutic gene is introduced into the culture using plasmids. After vector production the cells often need to be lysed to optimize the viral vector yield. At this stage the product is exposed to large quantities of nucleic acids from the host cell and residual plasmids.

DNA can form aggregates with product and cell debris, which makes purification challenging. Addition of DENARASE before the lysis step rapidly digests DNA without interacting with the product. This helps to improve performance of subsequent purification steps and increases the overall vector yield.

Get your free sample

  • Request your free sample!

    New customers from the pharmaceutical industry can request a free 100 kU sample for first lab trials. Please fill out the request form to get your free sample.

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Speak to a member of our expert team and learn more about our DENARASE products!

Qualified and used
by big pharma companies

  • „We have used DENARASE in our manufacturing production. It is good according to our test. We plan to use it in next valuable schedule.”

    US-Children Hospital, USA,
    Clinical Vector Core, Lead Scientist
  • „I tested the DENARASE yesterday and it was equally good as our standard in my setting. I will continue using it in variety of conditions that I normally use and I will most certainly be purchasing my future enzymes from you.”

    US University, USA,
  • „We are going to use DENARASE exclusively and no longer our usual nuclease. The product is excellent in performance and price.”

    US biopharmaceutical manufacturer, USA,
    Lab Manager Vector Production