Vaccines play an important role in the health of the world population. Some of their distinctive features is that they need to be available in large quantities, often very quickly and at a very low cost per unit. This results in a complicated supply chain - even more so because high quality is at least as important as for other pharma products, since vaccines are preventive rather than curative products.
Enzymes such as DENARASE endonuclease can be of great benefit for vaccine purification since they are available in large quantities at the highest quality level. Enzymes do not interfere with other molecules, which makes the process of developing urgently needed vaccines much faster.
Vaccines are intended to activate the human immune system against threats such as unknown viruses. The vaccines can be an inactivated form of the whole virus, an attenuated virus, or a part of the virus (antigen). More modern approaches even use viral vectors or directly introduce DNA or RNA in the body to express antigens in cells.
Endonucleases are used in a range of vaccine purification applications with various expression systems. Examples of processes with DENARASE are:
- Viral Vector Production, such as Adenoviruses and AAV’s
- Viral Vaccines
- Virus Like Particles
Treatment of cell lysates, before viral vector purification is one of the applications for which DENARASE is often used. High DNA concentrations originating from the host cell can form aggregates with the product and cell debris, which leads to reductions in capacity of purification equipment. Addition of DENARASE reduces the formation of aggregates, thus increasing the overall vaccine yield.