DENARASE ELISA Kit
The DENARASE ELISA Kit has been developed to monitor residual traces of Serratia marcescens endonucleases such as DENARASE® and Benzonase* in process samples of biopharmaceutical manufacturing processes. The kit may be used for both process development and quality control purposes.
The DENARASE® ELISA Kit uses an antibody sandwich principle in which the endonuclease is captured by two highly specific monoclonal antibodies. The first antibody is precoated on micro test plates and binds the endonucleases to the plate. After washing steps to remove excess unbound molecules, a second monoclonal antibody binds specifically to the captured endonuclease. This second antibody can be detected and quantified colorimetrically with the help of an enzyme conjugate and substrate solution.
Best in class performance
The carefully selected monoclonal antibodies provide an unmatched sensitivity and reproducibility in comparison to similar endonuclease ELISA Kits that work with polyclonal antibodies.
Endonuclease concentrations in final pharmaceutical product are often below the limit of detection of previous state-of-the-art kits. These values may raise uncertainty, as low signals as a result of material failure or human error cannot be excluded. However, with the DENARASE® ELISA Kit you can detect and quantify up to 30 times lower endonuclease concentrations compared to competitor ELISA Kits. This makes the assay extremely reliable, even when expected concentrations are at a very low level.
The highly specific monoclonal antibodies provide excellent reproducible results in both inter and intra assays. This enables an easy uptake in routine applications and avoids repeats in case of unclear results.
Broad working range
The broad working range of the Kit enables a convenient handling of samples as over-dilution is unlikely to happen.
Save up to 50% time compared to competitor kits.
*Benzonase Nuclease is a registered trademark of Merck KGaA