DENARASE®

For enzymatic DNA removal in Bioprocessing!

Order DENARASE® ELISA Kit now!

  • DENARASE® ELISA Kit - available now!

    For the quantitative analysis of residual Serratia marcescens endonucleases in process samples

    • The kit consists of a precoated micro test plate and ready to use reagents
    • The capacity of 1 kit is 480 measurements ​
    • Content of the kit (PDF-Download)
    • Estimated delivery time: 1–2 weeks (depending on region)​

    If you want buy in USD - click here!

    Buy in EUR

DENARASE ELISA Kit

Best in class performance
The carefully selected monoclonal antibodies provide an unmatched sensitivity and reproducibility in comparison to similar endonuclease ELISA Kits that work with polyclonal antibodies.

Best sensitivity
Endonuclease concentrations in final pharmaceutical product are often below the limit of detection of previous state-of-the-art kits. These values may raise uncertainty, as low signals as a result of material failure or human error cannot be excluded. However, with the DENARASE® ELISA Kit you can detect and quantify up to 30 times lower endonuclease concentrations compared to competitor ELISA Kits. This makes the assay extremely reliable, even when expected concentrations are at a very low level.

Best reproducibility
The highly specific monoclonal antibodies provide excellent reproducible results in both inter and intra assays. This enables an easy uptake in routine applications and avoids repeats in case of unclear results.

Broad working range
The broad working range of the Kit enables a convenient handling of samples as over-dilution is unlikely to happen.

Save time
Save up to 50% time compared to competitor kits.

*Benzonase Nuclease is a registered trademark of Merck KGaA

Features and benefits

Highest sensitivity

• Limit of Detection = 4 pg/mL
• Limit of Quantification = 12 pg/mL

• Reliably detect and quantify very low endonuclease levels in your final product
• Test the performance of individual DSP operations
Best reproducibility
(inter and intra assay, see also Prevalidation Report)
• Avoids repeats because of unclear results
• Easy uptake in routine applications
• Excellent linearity of dilution
50% Shorter assay time• Savings on manpower and equipment occupation
Broad working range
at low concentration		• More comfort with dilution of samples to meet the range
• Less risk of over-diluting the samples

Contents of the Kit

The DENARASE® ELISA Kit contains ready-to-use micro test plates and reagents for a total of 480 measurements. For details about the contents of the kit please refer to the table below.

No. 
ReagentDetailsQuantity
1Microtest plate
(ready-to-use)		96 wells (12 strips of 8 wells), pre-coated with a monoclonal

antibody specific for Serratia marcescens endonuclease

5 microplates
2Washing buffer
(10x)		Tris buffered saline containing surfactant and preservative, 10x concentrate3 x 100 mL
3Dilution buffer (10x)Tris buffered saline containing surfactant and preservative, 10x concentrate1 x 20 mL
4DENARASE Standard
(10 µg/mL)		DENARASE endonuclease in a buffered solution with preservative1 x 0.1 mL
5Detector antibody
(100x)		Monoclonal antibody specific for Serratia marcescens endonuclease, conjugated to
biotin, in a buffered solution with preservative, 100x concentrate		1 x 0.75 mL
6Enzyme conjugate
(100x)		Streptavidin-conjugated horseradish peroxidase in stabilized solution, 100x concentrate1 x 0.75 mL
7Substrate solutionTMB One Substrate solution, ready-to-use1 x 75 mL
8Stop solution0.5 M sulfuric acid1 x 75 mL

Order DENARASE® ELISA Kit now!

  • Buy ELISA Kit online

    The only kit that uses monoclonal antibodies enabling umatched accuracy & reproducibility.

    • The kit consists of a precoated micro test plate and ready to use reagents
    • The capacity of 1 kit is 480 measurements ​
    • Content of the kit (PDF-Download)
    • Estimated delivery time: 1–2 weeks (depending on region)​

    If you want buy in USD - click here!

    Buy in EUR

Get in touch with our DENARASE expert team!

Speak to a member of our expert team and learn more about our DENARASE products!