Enzyme Characteristics

Cofactor
DENARASE^® uses Mg²⁺ as a cofactor and a minimum amount is needed for enzyme activity. DENARASE^® requires 1- 2 mM Mg²⁺ cations for optimal activity (see Fig. 3). A large excess of MgCl₂reduces activity (see Fig. 4).

Stability and Storage Conditions
DENARASE^® is stable within the specification range at a storage temperature of -20 °C for a period of at least 24 months from the date of product release. Note: It is not recommended to store the product at -70 °C or below, since freezing the product will cause loss of activity.

Packaging Information
DENARASE^® is filled in non-pyrogenic, USP Class VI-compliant vials. The product vials are packed in polystyrene boxes and shipped under refrigerated conditions. Shipping temperature may differ from storage temperature without affecting product quality.

Please refer to the Validation Guide, which is available on request, for more information on the enzyme activity.

Effect of Temperature and pH value

Effect of Temperature on DENARASE performance

Fig. 1: Effect of temperature

The Effect of pH value in different buffer systems

Fig. 2: Effect of pH value in different buffer systems

The effect of low and high Magnesium Chloride

Product specification

In order to ensure a constant and high-quality level for DENARASE^®, each batch must fulfil the in-house acceptance criteria for the parameters listed below.

Criteria	Method	Specification
Appearance	visual	clear, transparent solution
Activity	photometric	>250 U/µL
Purity	Protein purity determined by SDS-PAGE and silver staining	≥99%
Specific Activity	Activity per protein content determined photometrically at 280 nm with a molar extinction coefficient of 44,600 L x mol^-1 x cm^-1	>6 x 10⁵U/mg
Protease activity	Protease detection assay	No protease activity detectable
Endotoxin level	LAL-Test acc. to Ph. Eur. 2.6.14, Method C	<0.25 EU/kU
Total microbial count	TAMC/TYMC acc. to Ph. Eur. 2.6.12	Aerobic bacteria: < 5 cfu/200 µL Yeast/moulds: < 5 cfu/200 µL

Unit-Definition: One unit (U) will digest salmon sperm DNA to acid-soluble
oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 min at pH 8.0 at 37 °C.

Order & test DENARASE now!

DENARASE, 100 kU

DENARASE, 500 kU

DENARASE, 1 MU

DENARASE, 5 MU

Free sample

ELISA Kit

DENARASE® 100 kU

Buy in EUR

Buy in USD

For process development of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%
Activity: > 250 U/µl
Formulation: buffered aqueous glycerol solution
Storage Temperature: -20°C
Estimated Delivery time: 1-2 weeks (depending on region)

Produced under GMP conditions and filled under ISO9001
Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
Depending on your application 10 – 60 U/ml is needed
net price excluding VAT and shipment costs

DENARASE®️ 500 kU

Buy in EUR

Buy in USD

For process development of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%
Activity: > 250 U/µl
Formulation: buffered aqueous glycerol solution
Storage Temperature: -20°C
Estimated Delivery time: 1-2 weeks (depending on region)

Produced under GMP conditions and filled under ISO9001
Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
Depending on your application 10 – 60 U/ml is needed
net price excluding VAT and shipment costs

DENARASE®️ 1 MU

For GMP manufacturing of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%
Activity: > 250 U/µl
Formulation: buffered aqueous glycerol solution
Storage Temperature: -20°C
Estimated Delivery time: 1-2 weeks (depending on region)

produced and filled under GMP conditions acc. to EU GMP regulation
Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
Depending on your application 10 – 60 U/ml is needed

DENARASE®️ 5 MU

For GMP manufacturing of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%
Activity: > 250 U/µl
Formulation: buffered aqueous glycerol solution
Storage Temperature: -20°C
Estimated Delivery time: 1-2 weeks (depending on region)

produced and filled under GMP conditions acc. to EU GMP regulation
Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
Depending on your application 10 – 60 U/ml is needed

Free sample DENARASE 25 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%
Activity: > 250 U/µl
Formulation: buffered aqueous glycerol solution
Storage Temperature: -20°C
Estimated Delivery time: 1-2 weeks (depending on region)

Produced under GMP conditions and filled under ISO9001
Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
Depending on your application 10 – 60 U/ml is needed
sample is shipped DAP (delivery at place)

ELISA Kit for Benzonase* and DENARASE®️

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For the quantitative analysis of residual Serratia marcescens endonucleases in process samples.

Save 20% on your first order with code ELISAKit_20

The kit consists of a pre-coated micro test plate and ready to use reagents.
The capacity of 1 kit is 480 measurements
Estimated Delivery time: 1-2 weeks (depending on region)
net price excluding VAT and shipment costs

*Benzonase Nuclease is a registered trademark of Merck KGaA

DENARASE^®

For enzymatic DNA removal in bioprocessing!