DENARASE®

For enzymatic DNA removal in bioprocessing!

Enzyme Characteristics

Cofactor
DENARASE® uses Mg2+ as a cofactor and a minimum amount is needed for enzyme activity. DENARASE® requires 1- 2 mM Mg2+ cations for optimal activity (see Fig. 3). A large excess of MgCl2 reduces activity (see Fig. 4).

Stability and Storage Conditions
DENARASE® is stable within the specification range at a storage temperature of -20 °C for a period of at least 24 months from the date of product release. Note: It is not recommended to store the product at -70 °C or below, since freezing the product will cause loss of activity.

Packaging Information
DENARASE® is filled in non-pyrogenic, USP Class VI-compliant vials. The product vials are packed in polystyrene boxes and shipped under refrigerated conditions. Shipping temperature may differ from storage temperature without affecting product quality.

Please refer to the Validation Guide, which is available on request, for more information on the enzyme activity.


Effect of Temperature and pH value

  • Effect of Temperature on DENARASE performance

    Fig. 1: Effect of temperature

  • The Effect of pH value in different buffer systems

    Fig. 2: Effect of pH value in different buffer systems

The effect of low and high Magnesium Chloride

Product specification

In order to ensure a constant and high-quality level for DENARASE®, each batch must fulfil the in-house acceptance criteria for the parameters listed below.

CriteriaMethodSpecification
Appearancevisualclear, transparent solution
Activityphotometric>250 U/µL
PurityProtein purity determined by SDS-PAGE and silver staining≥99%
Specific ActivityActivity per protein content determined photometrically at 280 nm
with a molar extinction coefficient of 44,600 L x mol-1 x cm-1
>6 x 105 U/mg
Protease activityProtease detection assayNo protease activity detectable
Endotoxin levelLAL-Test acc. to Ph. Eur. 2.6.14, Method C<0.25 EU/kU
Total microbial countTAMC/TYMC acc. to Ph. Eur. 2.6.12Aerobic bacteria: < 5 cfu/200 µL

Yeast/moulds: < 5 cfu/200 µL

Unit-Definition: One unit (U) will digest salmon sperm DNA to acid-soluble
oligonucleotides equivalent to a ΔA260nm of 1.0 in 30 min at pH 8.0 at 37 °C.

Order & test DENARASE now!

DENARASE, 100 kU
DENARASE, 500 kU
DENARASE, 1 MU
DENARASE, 5 MU
Free sample
ELISA Kit

DENARASE® 100 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%​
Activity:
> 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced under GMP conditions and filled under ISO9001
  • Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
  • Depending on your application 10 – 60 U/ml is needed ​
  • net price excluding VAT and shipment costs

DENARASE®️ 500 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%​
Activity:
> 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced under GMP conditions and filled under ISO9001
  • Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
  • Depending on your application 10 – 60 U/ml is needed ​
  • net price excluding VAT and shipment costs

DENARASE®️ 1 MU

For GMP manufacturing of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%​
Activity: > 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • produced and filled under GMP conditions acc. to EU GMP regulation
  • Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
  • Depending on your application 10 – 60 U/ml is needed ​

DENARASE®️ 5 MU

For GMP manufacturing of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.

Purity: ≥ 99%​
Activity: > 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • produced and filled under GMP conditions acc. to EU GMP regulation
  • Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
  • Depending on your application 10 – 60 U/ml is needed ​

Free sample DENARASE 25 kU

For process development of biologicals such as viral vectors and vaccines. The enzyme reduces all forms of DNA and RNA and enables a simpler, more efficient downstream process.


Purity: ≥ 99%​
Activity:
> 250 U/µl​
Formulation:
buffered aqueous glycerol solution
Storage Temperature:
-20°C
Estimated Delivery time:
1-2 weeks (depending on region)

  • Produced under GMP conditions and filled under ISO9001
  • Manufacturing process free of materials with TSE/BSE risk and of raw materials from animal origin
  • Depending on your application 10 – 60 U/ml is needed ​
  • sample is shipped DAP (delivery at place)

ELISA Kit for Benzonase* and DENARASE®️

For the quantitative analysis of residual Serratia marcescens endonucleases in process samples.

Save 20% on your first order with code ELISAKit_20

  • The kit consists of a pre-coated micro test plate and ready to use reagents. ​
  • The capacity of 1 kit is 480 measurements ​
  • Estimated Delivery time: 1-2 weeks (depending on region)​
  • net price excluding VAT and shipment costs

*Benzonase Nuclease is a registered trademark of Merck KGaA

Get in touch with our DENARASE expert team!

Vaishnavi Devarakonda
Vaishnavi Devarakonda DENARASE Sales +49 341 355 214 358
Dr. Christoph Plikat
Dr. Christoph Plikat DENARASE Sales +49 341 355 214 173
Dr. Kira Scheffler
Dr. Kira Scheffler DENARASE Sales +49 341 355 214 326

Speak to a member of our expert team and learn more about our DENARASE products!